Chemical Crosslinking Bioinks Customization Services
CD BioSciences is a biotechnology company offering the 3Dmicroflu™ technology platform for high resolution 3D bioprinting. We also offer a series of high-quality bioinks as well as an exclusive ink customisation service. Our bio-ink customisation is a professional, one-to-one and one-stop service that can meet as many of your experimental project needs as possible. In the following section you will find an introduction to our chemically cross-linked bioinks customisation services.
Fig. 1. Schematic diagram of polymer cross-linking based on a series of chemical methods.
Crosslinking is a method of transforming 3D printed bio-ink into a three-dimensional structure. Compared with physical crosslinking, chemical crosslinking forms crosslinking points that are usually strong enough to provide good mechanical properties. Chemical crosslinking achieves reaction departure and modulation by the action of external conditions, such as light, heat, high energy radiation, mechanical forces and ultrasound. Network formation in chemically cross-linked hydrogel bioinks can occur through a variety of chemical reactions including Schiff base coupling, hydrazine aldehyde coupling, Diels Alder bonding and azide alkyne cycloaddition. During the chemical reactions, covalent links are formed between the molecules, resulting in a network or bulk structure. Chemical crosslinking can be controlled by external factors and is therefore much easier and faster for customisation. The main issues we need to consider when designing and customising chemically cross-linked bioinks are the mechanical properties of the print, biocompatibility and control of external conditions. In general, the various types of chemically cross-linked bioinks tend to move towards a double or multiple cross-linked design concept. Our customized service will also adjust and improve print performance by introducing other types of materials in conjunction with the actual project requirements.
Customized bioinks for different chemical cross-linking methods at CD BioSciences
CD BioSciences' experienced team of experts has been providing high quality chemically cross-linked bioink design and customisation services, focusing on photocrosslinking bioinks, thermal crosslinking bioinks and enzymatic reaction crosslinking bioinks.
Photocrosslinking is achieved by light triggering various types of chain growth reactions culminating in the formation of a mesh-like three-dimensional structure, which is a process that cannot be achieved without light and photoinitiators. Photocrosslinking bioinks are easy to operate and can be controlled remotely at any time. CD BioSciences offers customisation services for all aspects of photo-crosslinking bioinks, such as the choice of crosslinker, photoinitiator type, photoinitiator concentration ratio and light intensity.
Thermal crosslinking is achieved by a process of heating or cooling the polymer solution and is mainly applied to polymers that can be heated or cooled continuously. In the design and customisation of thermally crosslinked bioinks, a series of issues need to be taken into account by CD BioSicences. Compared to other crosslinking methods, thermal crosslinking has a longer crosslinking time and the degree of crosslinking cannot be precisely controlled. In addition, we also need to consider that the heat generated by the heating process can have a negative impact on the cells in the bioink.
Enzymes can be used as catalysts to promote the formation of covalent bonds between protein-based polymers. Enzymatic crosslinking is the mildest and most physiologically appropriate of all crosslinking reactions, with good cell attachment and viability. For the design of enzymatic crosslinking bioinks, CD BioSciences has focused on improving mechanical properties. In general, we improve the printability of enzymatic reactions by means of double cross-linking.
Based on a high-resolution 3Dmicroflu™ 3D printing technology platform and an experienced team of experts, CD BioSciences can offer you high-quality customisation services for special bio-inks. After placing your order, you will receive a one-stop shop for bio-ink customisation from CD BioSciences. We always insist on meeting your experimental needs as much as possible, with the main objective of facilitating and advancing your 3D printing process.
If you are interested in our bioinks customization services, please contact us now for exclusive services. All services are available on a 24/7/365 basis.
For research use only, not intended for any clinical use.
